akt pathway inhibitor mk2206  (Biogems International)

Journal: iScience

Article Title: Broad and diverse roles of sphingosine-1-phosphate/sphingosine-1-phosphate receptors in the prostate

doi: 10.1016/j.isci.2024.111290

Figure Lengend Snippet: MK2206 and U0126 rescued the alterations induced by S1P and S1PR1/3 overexpression (A and C) Statistical analysis of percentages (%) of cells at each stage. (B and D) Western blot assay of cell cycle- and pathway-related proteins. Data are represented as M ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ns means no significant difference.

Article Snippet: ERK1/2 pathway inhibitor U0126 (Cat. HY-12031A) and AKT pathway inhibitor MK2206 (Cat. HY-108232) were purchased from MCE.

Techniques: Over Expression, Western Blot

Journal: iScience

Article Title: Broad and diverse roles of sphingosine-1-phosphate/sphingosine-1-phosphate receptors in the prostate

doi: 10.1016/j.isci.2024.111290

Figure Lengend Snippet:

Article Snippet: ERK1/2 pathway inhibitor U0126 (Cat. HY-12031A) and AKT pathway inhibitor MK2206 (Cat. HY-108232) were purchased from MCE.

Techniques: Recombinant, SYBR Green Assay, Staining, CCK-8 Assay, Contraction Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: Advanced Healthcare Materials

Article Title: 3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and Functionalization

doi: 10.1002/adhm.202402606

Figure Lengend Snippet: Immunostaining for SSC markers following the replacement of the neonatal mouse SSC growth factor EGF with Akt‐inhibitor MK2206 in hiPSC‐SSC cultures.

Article Snippet: SSCs were expanded on a sulfated dextran‐4‐armed polyethylene glycol (starPEG) hydrogel matrix functionalized with vitronectin and fibronectin type II domain (FN2) peptide motifs (denovoMATRIX, GmbH) in Human Plasma‐Like Medium (HPLM), 15% Cell Therapy Systems (CTS) KnockOut Serum Replacement (SR) XenoFree supplement (Gibco, 12 618 012), 1 μM vitamin C (Millipore Sigma, A4403), 1 μM vitamin E (Millipore Sigma, T1157), 10 μg mL −1 biotin (Millipore Sigma, B4639), 60 ng mL −1 progesterone (Millipore Sigma, P8783), 60 μM putrescine (Millipore Sigma, P5780), 30 μg mL −1 pyruvate (Millipore Sigma, S8636), 30 ng mL −1 β‐estradiol (Sigma, E2758), 1X Minimum Essential Medium (MEM) Vitamin Solution (Gibco, 11 120 052), 1X GlutaMAX Supplement (Gibco, 35 050 061), 1 μL mL −1 DL‐lactate (Millipore Sigma, L4263), 50 μM β‐mercaptoethanol (Gibco, 31350‐010), 15 ng mL −1 Glial Cell‐Derived Neurotrophic Factor (GDNF, Peprotech, AF‐450‐010), 10 ng mL −1 heat stable FGF2, and either 10 ng mL −1 EGF or 100 nM Akt pathway inhibitor MK2206 (Biogems, 1 031 320).

Techniques: Immunostaining

Journal: Aging (Albany NY)

Article Title: PTEN loss regulates alveolar epithelial cell senescence in pulmonary fibrosis depending on Akt activation

doi: 10.18632/aging.102262

Figure Lengend Snippet: Inhibition of Akt activation attenuates AEC senescence. Inhibitor (20 μm/L LY294002 or 5 μg/ml MK2206) of the AKT pathway was added 1 hour before bleomycin (10 μg/ml). After 72 hours, the medium was changed to fresh medium for 24 hours. AKT inactivation and P21 WAF1 expression induced by LY294002 ( A – C ) or MK2206 ( F – H ) were detected by western blotting. Cellular senescence after inhibitor LY294002 ( D , E ) or MK2206 ( I, J ) pretreatment was analyzed by SA-β-Gal staining. Data are shown as the mean ± SEM, n ≥ 3 per group. * p < 0.05. One-way ANOVA followed by Dunnett’s Multiple Comparison Test.

Article Snippet: MK2206, an Akt pathway inhibitor, was purchased from Selleckchem, dissolved in DMSO (Sigma, USA) and stored at −20°C.

Techniques: Inhibition, Activation Assay, Expressing, Western Blot, Staining

Journal: Aging (Albany NY)

Article Title: PTEN loss regulates alveolar epithelial cell senescence in pulmonary fibrosis depending on Akt activation

doi: 10.18632/aging.102262

Figure Lengend Snippet: Akt inactivation rescues AEC senescence in vitro. After PTEN was stably knocked down, an inhibitor of the AKT pathway (20 μm/L LY294002 or 5 μg/ml MK2206) was added 1 hour before bleomycin (10 μg/ml) to A549 cells for 72 hours followed by a fresh medium transfer for 24 hours. ( A , B ) Rescue of cell senescence by LY294002 was determined by SA-β-Gal staining (original magnification, 200×), and decreased expression of P21 WAF1 ( C , D ) was confirmed by western blot. Rescue of cell senescence by MK2206 was determined by SA-β-Gal staining ( E , F ) and P21 WAF1 expression ( G , H ). Data are shown as the mean ± SEM, n ≥ 3 per group. * p < 0.05, ** p < 0.01. One-way ANOVA followed by Dunnett’s Multiple Comparison Test.

Article Snippet: MK2206, an Akt pathway inhibitor, was purchased from Selleckchem, dissolved in DMSO (Sigma, USA) and stored at −20°C.

Techniques: In Vitro, Stable Transfection, Staining, Expressing, Western Blot